For the rst time a new ecient analytical approach based on microbore AF-HPLC hyphenated to ICP-sector eld MS is proposed for the simultaneous speciation analysis of the selenoproteins glutathione peroxidase, selenoprotein P and seleno-albumin in human serum without prior sam- ple preparation or mathematical correction of the spectral interferences. Microbore AF-HPLC allows the reduction of sample volume to a single micro-drop (5 L) and the decrease of chro- matographic run time to 7 min. A high-eciency sample introduction system is also proposed to achieve an enhancement in sensitivity of more than one order of magnitude.

Fast speciation analysis of selenoproteins in a micro-drop of human serum by microbore affinity-HPLC hyphenated to ICP-sector field-MS

ROMAN, Marco
Methodology
;
COZZI, Giulio
Membro del Collaboration Group
;
CESCON, Paolo
Membro del Collaboration Group
;
BARBANTE, Carlo
Supervision
2009

Abstract

For the rst time a new ecient analytical approach based on microbore AF-HPLC hyphenated to ICP-sector eld MS is proposed for the simultaneous speciation analysis of the selenoproteins glutathione peroxidase, selenoprotein P and seleno-albumin in human serum without prior sam- ple preparation or mathematical correction of the spectral interferences. Microbore AF-HPLC allows the reduction of sample volume to a single micro-drop (5 L) and the decrease of chro- matographic run time to 7 min. A high-eciency sample introduction system is also proposed to achieve an enhancement in sensitivity of more than one order of magnitude.
European Winter Conference on Plasma Spectrochemistry 2009
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10278/3641346
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